Warm-core eddies detached from the Loop Current play a crucial role in transporting substantial heat from the Caribbean to the Gulf of Mexico. Using satellite altimetric observations, the drivers of the detachment and separation of Loop Current eddies were revealed. A notably elongated Loop Current and the intensified presence of cyclonic eddies west of the Florida Shelf are key factors in the process of eddy detachment. Conversely, for the separation of these eddies, it is crucial that intensified cyclonic eddies occur on both sides of the Loop Current. The co-occurrence of eastern and western cyclonic eddies in the Loop Current bottleneck zone leads to the formation of a large cyclonic structure. The latter seems to prevent newly formed Loop Current eddies from reattaching and can limit the growth of the Loop Current during several months. These findings underscore the crucial role of cyclonic eddies in regulating the detachment and separation of Loop Current eddies.