Seismic oceanography acquires water column reflections by seismic exploration. The use of a high-frequency seismic source such as sparker can enhance the resolution of reflection image but suffers from a low signal-to-noise ratio problem. In this study, we applied a machine learning to remove the random noise in water column seismic section. We constructed appropriate training data and showed that the machine learning can successfully remove the random noise in the water column seismic section.