Articles | Volume 11, issue 6
https://doi.org/10.5194/os-11-947-2015
https://doi.org/10.5194/os-11-947-2015
Technical note
 | 
07 Dec 2015
Technical note |  | 07 Dec 2015

Technical note: Could benzalkonium chloride be a suitable alternative to mercuric chloride for preservation of seawater samples?

J. Gloël, C. Robinson, G. H. Tilstone, G. Tarran, and J. Kaiser

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Cited articles

Anderson, L. A. and Sarmiento, J. L.: Redfield ratios of remineralization determined by nutrient data analysis, Global Biogeochem. Cy., 8, 65–80, https://doi.org/10.1029/93GB03318, 1994.
Block, S. S.: Disinfection, Sterilization and Preservation, Lippincott Williams & Wilkins, Philadelphia, USA, 2001.
Craig, H. and Hayward, T.: Oxygen supersaturation in the ocean: biological versus physical contributions, Science, 235, 199–202, 1987.
Dickson, A. G., Sabine, C. L., and Christian, J. R.: Guide to best practices for ocean CO2 measurements, PICES Special Publication 3, IOCCP Report No. 8, 191 pp., 2007.
Emerson, S., Quay, P., Stump, C., Wilbur, D., and Know, M.: O2, Ar, N2, and 222Rn in surface waters of the subarctic ocean: net biological O2 production, Global Biogeochem. Cy., 5, 49–69, 1991.
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Short summary
We assess benzalkonium chloride (BAC) as alternative to mercuric chloride (HgCl2) for preservation of seawater samples. BAC concentrations of 50mg dm–3 inhibited microbial activity for at least 3 days in samples tested with chlorophyll a concentrations up to 1mg m–3. With fewer risks to health and environment, and lower waste disposal costs, BAC could be a short-term alternative to HgCl2, but cannot replace it for oxygen triple isotope samples, which require storage over weeks to months.
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