High-resolution monitoring of marine protists based on an observation strategy integrating automated on-board filtration and molecular analyses

Metfies, Katja; Schroeder, Friedhelm; Hessel, Johanna; Wollschläger, Jochen; Micheller, Sebastian; Wolf, Christian; Kilias, Estelle; Sprong, Pim; Neuhaus, Stefan; Frickenhaus, Stephan; Petersen, Wilhelm

doi:https://doi.org/10.5194/os-12-1237-2016

Articles | Volume 12, issue 6

https://doi.org/10.5194/os-12-1237-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Special issue:

COSYNA: integrating observations and modeling to understand...

https://doi.org/10.5194/os-12-1237-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 12, issue 6

Research article

|

22 Nov 2016

Research article |

| 22 Nov 2016

High-resolution monitoring of marine protists based on an observation strategy integrating automated on-board filtration and molecular analyses

Katja Metfies, Friedhelm Schroeder, Johanna Hessel, Jochen Wollschläger, Sebastian Micheller, Christian Wolf, Estelle Kilias, Pim Sprong, Stefan Neuhaus, Stephan Frickenhaus, and Wilhelm Petersen

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Final revised paper (published on 22 Nov 2016)
Preprint (discussion started on 23 May 2016)

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'reviewer comments', Anonymous Referee #1, 22 Jun 2016
- AC1: 'Reply to reviewer comments, Anonymous Referee #1', Katja Metfies, 30 Aug 2016
RC2: 'Revewer comments to Metfies et al. (2016)', Anonymous Referee #2, 08 Aug 2016
- AC2: 'Reply to reviewer comments, Anonymous Referee #2', Katja Metfies, 30 Aug 2016

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by Katja Metfies on behalf of the Authors (30 Aug 2016) Author's response Manuscript

ED: Publish as is (12 Sep 2016) by Oliver Zielinski

AR by Katja Metfies on behalf of the Authors (31 Oct 2016) Author's response Manuscript

Short summary

Here we introduce a new molecular-based observation strategy for high-resolution assessment of marine microbes (e.g., microalgae) in space and time. The observation strategy combines automated sampling on board ships or observation platforms with a variety of different molecular genetic methods for refined observation of marine microbes at adaquate scales, in order to better understand the impact of climate change on this group of organisms, which are at the base of marine food webs.