Articles | Volume 11, issue 1
https://doi.org/10.5194/os-11-83-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/os-11-83-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Research article
| 
13 Jan 2015
Research article |
| 13 Jan 2015
An alternative method for correcting fluorescence quenching
L. Biermann
CORRESPONDING AUTHOR
Sea Mammal Research Unit, Scottish Oceans Institute, St. Andrews, UK
C. Guinet
Centre National de la Recherche Scientifique, Centre d'Etudes Biologiques de Chizé, Villiers en Bois, France
M. Bester
Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Pretoria, South Africa
A. Brierley
Pelagic Ecology Research Group, Scottish Oceans Institute, St. Andrews, UK
L. Boehme
Sea Mammal Research Unit, Scottish Oceans Institute, St. Andrews, UK
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26 citations as recorded by crossref.
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- Subsurface chlorophyll maxima reduce the performance of non-photochemical quenching corrections in the Southern Ocean K. Baldry et al. 10.3389/fmars.2023.1302999
- When Mixed Layers Are Not Mixed. Storm‐Driven Mixing and Bio‐optical Vertical Gradients in Mixed Layers of the Southern Ocean M. Carranza et al. 10.1029/2018JC014416
- A comparison of chlorophyll a values obtained from an autonomous underwater vehicle to satellite-based measures for Lake Michigan D. Bennion et al. 10.1016/j.jglr.2019.04.003
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- Validation of Non-photochemical Quenching Corrections for Chlorophyll-a Measurements Aboard Ships of Opportunity H. Travers-Smith et al. 10.3389/fmars.2021.686750
- Using machine learning to correct for nonphotochemical quenching in high‐frequency, in vivo fluorometer data M. Lucius et al. 10.1002/lom3.10378
- Chlorophyll a estimation in lakes using multi-parameter sonde data X. Liu & A. Georgakakos 10.1016/j.watres.2021.117661
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23 citations as recorded by crossref.
- A chlorophyll a, non‐photochemical fluorescence quenching correction method for autonomous underwater vehicles in shelf sea environments C. Mitchell et al. 10.1002/lom3.10597
- Autonomous profiling float observations of the high-biomass plume downstream of the Kerguelen Plateau in the Southern Ocean M. Grenier et al. 10.5194/bg-12-2707-2015
- An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms S. Thomalla et al. 10.1002/lom3.10234
- Animal Borne Ocean Sensors – AniBOS – An Essential Component of the Global Ocean Observing System C. McMahon et al. 10.3389/fmars.2021.751840
- Subsurface Chlorophyll-a Maxima in the Southern Ocean K. Baldry et al. 10.3389/fmars.2020.00671
- Improved correction for non-photochemical quenching of in situ chlorophyll fluorescence based on a synchronous irradiance profile X. Xing et al. 10.1364/OE.26.024734
- Global distribution and variability of subsurface chlorophyll a concentrations S. Yasunaka et al. 10.5194/os-18-255-2022
- Application of a new net primary production methodology: a daily to annual-scale data set for the North Sea, derived from autonomous underwater gliders and satellite Earth observation B. Loveday et al. 10.5194/essd-14-3997-2022
- Diel quenching of Southern Ocean phytoplankton fluorescence is related to iron limitation C. Schallenberg et al. 10.5194/bg-17-793-2020
- The impact of high rainfall events on the submesoscale salinity field in a coastal sea: Greater Cook Strait, New Zealand K. Jhugroo et al. 10.1080/00288330.2024.2368853
- Deriving a Proxy for Iron Limitation From Chlorophyll Fluorescence on Buoyancy Gliders T. Ryan-Keogh & S. Thomalla 10.3389/fmars.2020.00275
- Phytoplankton biomass and photophysiology at the sub-Antarctic Prince Edward Islands ecosystem in the Southern Ocean T. Lamont et al. 10.1016/j.jmarsys.2021.103669
- Subsurface algal blooms of the northwestern Arabian Sea S. Piontkovski et al. 10.3354/meps11990
- Subsurface chlorophyll maxima reduce the performance of non-photochemical quenching corrections in the Southern Ocean K. Baldry et al. 10.3389/fmars.2023.1302999
- When Mixed Layers Are Not Mixed. Storm‐Driven Mixing and Bio‐optical Vertical Gradients in Mixed Layers of the Southern Ocean M. Carranza et al. 10.1029/2018JC014416
- A comparison of chlorophyll a values obtained from an autonomous underwater vehicle to satellite-based measures for Lake Michigan D. Bennion et al. 10.1016/j.jglr.2019.04.003
- Diurnal regulation of photosynthetic light absorption, electron transport and carbon fixation in two contrasting oceanic environments N. Schuback & P. Tortell 10.5194/bg-16-1381-2019
- Fluorescence characteristics in the deep waters of South Gulf of México I. Schifter et al. 10.1016/j.marpolbul.2017.09.001
- Validation of Non-photochemical Quenching Corrections for Chlorophyll-a Measurements Aboard Ships of Opportunity H. Travers-Smith et al. 10.3389/fmars.2021.686750
- Using machine learning to correct for nonphotochemical quenching in high‐frequency, in vivo fluorometer data M. Lucius et al. 10.1002/lom3.10378
- Chlorophyll a estimation in lakes using multi-parameter sonde data X. Liu & A. Georgakakos 10.1016/j.watres.2021.117661
- Chlorophyll fluorescence as measured in situ by animal-borne instruments in the northeastern Pacific Ocean T. Keates et al. 10.1016/j.jmarsys.2019.103265
- Towards a Multi‐Platform Assimilative System for North Sea Biogeochemistry J. Skákala et al. 10.1029/2020JC016649
3 citations as recorded by crossref.
- On the vertical distribution of the chlorophyll a concentration in the Mediterranean Sea: a basin-scale and seasonal approach H. Lavigne et al. 10.5194/bg-12-5021-2015
- Operational use of continuous surface fluorescence measurements offshore Rimini to validate satellite-derived chlorophyll observations E. Böhm et al. 10.1080/1755876X.2015.1117763
- Development and field testing a satellite-linked fluorometer for marine vertebrates M. Lander et al. 10.1186/s40317-015-0070-7
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Latest update: 23 Feb 2025
Short summary
To protect from light stress, phytoplankton inhibit photosynthesis and suppress fluorescence through the process of quenching. This makes them invisible to fluorometers. Conventionally, quenching is corrected by taking maximum fluorescence yield in a surface mixed layer (MLD) and filling in the invisible proportion. This is only valid in waters where turbulence is high and phytoplankton are uniformly mixed. Here, we show that correcting from Zeu is a robust alternative to correcting from MLD
To protect from light stress, phytoplankton inhibit photosynthesis and suppress fluorescence...
